Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
Channel Rhodopsins01:11

Channel Rhodopsins

Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiology report quality improvement through structured provider feedback: a data-driven initiative.

Abdominal radiology (New York)Ā·2026
Same author

Landscape and biogenesis of piRNAs in HBV-associated hepatocarcinogenesis: from repetitive elements to oncogenic circuits.

Discover oncologyĀ·2026
Same author

DOCTER: a genetically encoded switchable protein module for ERĪ±-mediated transcriptional regulation.

Gene therapyĀ·2026
Same author

Blood cancer differentiation based on IR spectroscopy and chemometrics.

Computer methods and programs in biomedicineĀ·2026
Same author

Effects of hydrostatic pressure on epithelial dome formation and stability.

Soft matterĀ·2026
Same author

Optogenetic manipulation of estrogen receptor signaling to improve estrogen deficiency.

iScienceĀ·2026
Same journal

Stretching the limits: Decoding hidden biology with vibrational probes.

Current opinion in chemical biologyĀ·2026
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biologyĀ·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biologyĀ·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biologyĀ·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biologyĀ·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biologyĀ·2026
See all related articles

Related Experiment Video

Updated: May 27, 2026

Dynamic Light-Induced Protein Patterns at Model Membranes
07:10

Dynamic Light-Induced Protein Patterns at Model Membranes

Published on: February 23, 2024

Using light to see and control membrane traffic.

Yingke Xu1, Thomas J Melia, Derek K Toomre

  • 1Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520-8002, USA.

Current Opinion in Chemical Biology
|November 15, 2011
PubMed
Summary
This summary is machine-generated.

Superresolution microscopy advances enable visualization of cellular membrane trafficking. New dye switching techniques and light-controlled methods offer enhanced multicolor imaging and manipulation of these dynamic processes.

More Related Videos

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

Related Experiment Videos

Last Updated: May 27, 2026

Dynamic Light-Induced Protein Patterns at Model Membranes
07:10

Dynamic Light-Induced Protein Patterns at Model Membranes

Published on: February 23, 2024

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
11:31

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

Published on: May 12, 2023

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Cellular compartmentalization relies on membrane trafficking, including vesiculation and tubulation.
  • Superresolution imaging techniques are crucial for observing these dynamic cellular events.
  • Current nanoscopy methods are often limited by the ON/OFF switching properties of fluorescent dyes.

Purpose of the Study:

  • To explore advanced nanoscopy techniques for studying membrane trafficking.
  • To expand the capabilities of multicolor imaging in superresolution microscopy.
  • To introduce novel light-controlled methods for manipulating membrane dynamics.

Main Methods:

  • Utilizing ground state depletion to switch dyes into long-lived dark states.
  • Exploiting dye photophysical properties (redox potential, pKa) for enhanced imaging.
  • Employing homodimerization, heterodimerization, and selective release technologies.

Main Results:

  • Demonstrated expansion of nanoscopy probe repertoire for multicolor imaging.
  • Showcased exploitation of specific dye photophysics for improved imaging.
  • Highlighted new technologies for light-controlled manipulation of membrane trafficking.

Conclusions:

  • Advanced dye switching and light-controlled strategies hold significant promise for studying membrane trafficking.
  • These techniques offer enhanced capabilities for multicolor imaging and dynamic process manipulation.
  • Current limitations of these novel strategies warrant further investigation and development.