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Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

A high-throughput microchromatography platform for quantitative analytical scale protein sample preparation.

Scott Fulton¹, Steve Murphy, Jenn Reich

¹BioSystem Development, LLC, Madison, WI 53717, USA. scott.fulton@biosystemdevelopment.com

Journal of Laboratory Automation

|November 19, 2011

Summary

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This study introduces a high-throughput microchromatography platform for rapid protein purification. The system enables parallel processing of numerous samples, significantly streamlining protein analysis workflows.

Area of Science:

Biochemistry
Analytical Chemistry
Biotechnology

Background:

Protein analysis often involves extensive sample preparation, including purification from complex matrices.
Current methods can be time-consuming and require large sample volumes.

Purpose of the Study:

To develop a high-throughput microchromatography platform for efficient protein purification.
To reduce sample preparation time and improve the scalability of protein analysis.

Main Methods:

A novel platform utilizing disposable micro-cartridge devices with small packed resin beds.
Parallel processing of 96+ samples using a modified 96-channel liquid handler with precise flow control.
Application of affinity or physical chromatography for quantitative protein purification.

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Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Main Results:

Capable of processing 96 or more small volume samples in parallel.
Purification yields ranging from 10 pg to 100 μg of protein per sample.
Enabled 30-minute immunoassays using standard reagents and developed enzymatic digestion methods for glycan profiling.

Conclusions:

The developed platform significantly enhances throughput for protein purification.
It offers a versatile solution for various protein analysis applications, including immunoassays and glycan profiling.
This technology streamlines complex sample preparation, accelerating research and diagnostics.