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Updated: May 27, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Optimization of plant des-pGlu1-Brazzein gene according to yeasty biased codons and its expression in Pichia
Chunli Li1, Lu Han, Zhenyu Zheng
1College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China. hnclli@163.com
Abstract:
According to the amino acid sequence of des-pGlu1-Brazzein, 4 pairs of oligonucleotide with cosmic site were synthesized by using yeasty biased codons. After linkage and PCR, the 179 bp code area of des-pGlu1-Brazzein was obtained and inserted into pPIC9K, which resulted in the recombinant expression vector pPIC9K-Bra. By digestion with Sal I, the lined pPIC9K-Bra was transformed into Pichia pastoris GS115 by electric shock. The results of expression indicted that the secreted target protein accounted for 51.6% of total protein in the supernatant and showed biological activity after purification.
