[Construction and expression characterization of transgenic chicken bioreactor vector]

Area of Science:

• Biotechnology
• Genetic Engineering
• Molecular Biology

Context:

• Developing efficient bioreactors is crucial for producing valuable biomolecules.
• Transgenic animal models offer unique advantages for biopharmaceutical production.
• The chicken oviduct presents a potential site for large-scale protein expression.

Purpose:

• To construct and evaluate transgenic chicken bioreactor vectors.
• To compare the efficacy and specificity of chicken ovalbumin promoter, lentiviral, and CMV promoter-driven expression systems.
• To assess reporter gene expression in various cell types.

Summary:

• Transgenic vectors were created using chicken ovalbumin promoter, lentiviral, and CMV promoter systems with GFP and luciferase reporters.
• Expression was tested in chicken oviduct epithelial cells, fibroblasts, mouse 3T3-L1 cells, and bovine mammary cells.
• Chicken ovalbumin promoter showed low activity and lacked cell-type specificity, unlike lentiviral vectors which demonstrated efficient and broad expression.

Impact:

• The chicken ovalbumin promoter is unsuitable for highly efficient and specific transgenic chicken bioreactors.
• Lentiviral vectors offer a promising alternative for developing chicken oviduct bioreactors.
• This research provides insights into vector design for biotechnological applications in avian systems.