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Updated: May 27, 2026

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
Published on: July 12, 2013
Tissue imaging using nanospray desorption electrospray ionization mass spectrometry.
Julia Laskin1, Brandi S Heath, Patrick J Roach
1Chemical and Materials Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA. Julia.Laskin@pnnl.gov
A new ambient ionization technique, nanospray desorption ionization (nano-DESI), achieves high-resolution chemical imaging of biological tissues. This method offers improved spatial resolution for mass spectrometry applications.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Molecular Biology
Background:
- Ambient ionization imaging mass spectrometry enables detailed chemical analysis of biological samples in their native state.
- Current methods are limited by ion transfer efficiency, restricting spatial resolution.
Purpose of the Study:
- To introduce and evaluate nanospray desorption ionization (nano-DESI) for ambient imaging mass spectrometry.
- To demonstrate nano-DESI's capability for high-resolution, sensitive chemical characterization of biological tissues.
Main Methods:
- Utilized a novel nano-DESI probe with controlled solvent delivery for desorption and self-aspirating nanospray for ionization.
- Applied the technique to analyze tissue samples directly without prior preparation.
Main Results:
- Achieved spatially resolved analysis of biological samples with high sensitivity.
- Demonstrated a spatial resolution better than 12 micrometers using nano-DESI imaging.
- Confirmed the potential for improved detection efficiency compared to existing methods.
Conclusions:
- Nano-DESI is a promising new technique for ambient imaging mass spectrometry.
- The enhanced spatial resolution and sensitivity of nano-DESI open new avenues in clinical diagnostics, drug discovery, and biological research.
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