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Updated: May 27, 2026
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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
CCR5 promoter haplotype transcription complex characterization.
Daifeng Jiang1, Srinivas Mummidi, Sunil K Ahuja
1Department of Chemistry, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249, USA. daifeng.jiang@utsa.edu
Journal of Health Care for the Poor and Underserved
|November 22, 2011
Summary
Differences in CCR5 promoter DNA sequences (haplotypes) affect how transcription factors bind, influencing human immunodeficiency virus (HIV) susceptibility and acquired immune deficiency syndrome (AIDS) progression.
Area of Science:
- Genetics
- Virology
- Molecular Biology
Background:
- CC chemokine receptor 5 (CCR5) is crucial for human immunodeficiency virus (HIV) cell entry and replication.
- Single nucleotide polymorphisms (SNPs) in the CCR5 promoter regulate its expression and are linked to HIV susceptibility.
Purpose of the Study:
- To identify transcription factors (TFs) that differentially bind to two distinct CCR5 promoter haplotypes (HHA and HHE).
- To understand how these differential TF-haplotype interactions influence HIV pathogenesis.
Main Methods:
- Utilized promoter trapping and 2D-southwestern blot to isolate transcription complexes.
- Employed HPLC-ESI-MS/MS to identify proteins specifically bound to CCR5 haplotypes.
Main Results:
- Demonstrated clear differences in TF binding profiles between CCR5 HHA and HHE haplotypes.
- Identified specific proteins that bind differentially to the polymorphic sites.
Conclusions:
- CCR5 haplotype variations significantly alter TF binding, impacting CCR5 expression.
- This finding provides a molecular basis for understanding variations in HIV susceptibility and AIDS progression.
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