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Updated: May 27, 2026

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
Published on: July 27, 2015
Intersession reliability of the electromyographic signal during incremental cycle ergometry: quadriceps femoris
Liesle A Travis1, Steven J Arthmire, Ahmed M Baig
1Integrative Physiology of Exercise Laboratory, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, Detroit, Michigan 48201, USA.
Introduction:
The purposes of this investigation were to determine the: (1) reproducibility of the patterns of responses for electromyographic (EMG) amplitude and mean power frequency (MPF); and (2) intraclass correlation coefficient (ICC) of the EMG amplitude and MPF during three incremental cycle ergometer tests separated by 48 hours.
Methods:
Ten men performed incremental cycle ergometry tests to exhaustion on three separate occasions. Surface EMG signals were recorded simultaneously from the three superficial quadriceps muscles at each trial.
Results:
Polynomial regression indicated that, for >95% of the cases, the best-fit model was the same at each trial for EMG amplitude but not for EMG MPF. The ICC values were high for EMG amplitude but low for EMG MPF.
Conclusions:
These results indicate that EMG amplitude is a reliable measure of motor unit activation strategy during incremental cycle ergometry, whereas the EMG MPF was unreliable.
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