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Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
Published on: November 17, 2018
Phenanthroline-based magnetic nanoparticles as a general agent to bind histidine-tagged proteins
Eun Jin Cho1, Hyun Joo Kim, Younho Song
1Department of Wood Science and Landscape Architecture, BK21 Program, Bio-energy Research Institute, Chonnam National University, Gwangju, 500-757, S. Korea.
Abstract:
We herein report the preparation of PSMN (phenanthroline-based silica coated magnetic nanoparticles), and their applications for protein purification as selective magnetic probes of histidine-tagged proteins in cell lysates. This simple system serves as a useful alternative to existing protocols for his-tagged protein separation and as a versatile agent for transporting and anchoring proteins.
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