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Updated: May 27, 2026

Technique of Conjunctival Biopsy and Direct Immunofluorescence for Diagnosing Mucous Membrane Pemphigoid
Published on: June 17, 2025
Biopsy techniques and diagnoses & treatment of mucocutaneous lesions
Michael H Chan1, Joshua C Wolf
1Oral & Maxillofacial Surgery/Dental Service, Department of Veterans Affairs, New York Harbor Healthcare Systems (Brooklyn Campus), 800 Poly Place (Bk-160), Brooklyn, NY 11209, USA. Michael.chan2@va.gov
Abstract:
Oral mucosal lesions are commonly encountered in clinical practice. One study reported that they occurred in approximately 27.9% of patients aged 17 years and older and in 10.3% of children aged 2 to 17 years. The diagnosis and treatment of mucosal diseases should be an integral part of the general practitioner's practice. According to an American Dental Association survey conducted in 2007, 44% of biopsies were performed by a general practictioner. Understanding of the fundamentals of diagnosing mucocutaneous lesions requires a sound knowledge of their origin and clinical course, and of biopsy methods using contemporary diagnostic tools and techniques.
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