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Hypothalamic Ahi1 mediates feeding behavior through interaction with 5-HT2C receptor
Hao Wang1, Zhenbo Huang, Liansha Huang
1CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
The Journal of Biological Chemistry
|November 30, 2011
Summary
Abelson helper integration site 1 (Ahi1) protein regulates feeding behavior by interacting with serotonin receptor 2C (5-HT(2C)R). This interaction influences energy balance and appetite control via the serotonin signaling pathway.
Area of Science:
- Neuroscience
- Molecular Biology
- Endocrinology
Background:
- Brain signaling pathways regulate feeding and energy balance.
- Key molecular players in these pathways are not fully identified.
Purpose of the Study:
- To investigate the role of Abelson helper integration site 1 (Ahi1) in feeding behavior.
- To explore the interaction between Ahi1 and serotonin receptor 2C (5-HT(2C)R).
Main Methods:
- Demonstrated co-localization and interaction between hypothalamic Ahi1 and 5-HT(2C)R.
- Investigated the effect of fasting on Ahi1 and 5-HT(2C)R expression.
- Performed knockdown of hypothalamic Ahi1.
- Analyzed neuropeptide Y and proopiomelanocortin expression.
Main Results:
- Ahi1 interacts with and promotes lysosomal degradation of 5-HT(2C)R.
- Fasting increases hypothalamic Ahi1 expression and decreases 5-HT(2C)R expression.
- Ahi1 knockdown increases 5-HT(2C)R expression, reduces food intake and body weight.
- Ahi1 regulates neuropeptide Y and proopiomelanocortin expression.
Conclusions:
- Ahi1 mediates feeding behavior through interaction with 5-HT(2C)R.
- Ahi1 modulates the serotonin signaling pathway to regulate appetite and energy balance.
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