Nucleic acid amplification testing in suspected child sexual abuse
Debra Esernio-Jenssen1, Marilyn Barnes
1University of Florida, Gainesville, 32607, USA. dejenssen@peds.ufl.edu
Journal of Child Sexual Abuse
|December 1, 2011
Summary
Nucleic acid amplification testing offers a sensitive, specific, and less invasive method for detecting sexually transmitted infections in children. This approach may be a more effective alternative to traditional cultures for sexually abused youth.
Area of Science:
- Pediatrics
- Infectious Diseases
- Forensic Medicine
Background:
- The American Academy of Pediatrics advises site-specific cultures for sexually victimized children.
- Current diagnostic methods for sexually transmitted infections (STIs) in children can be invasive.
Purpose of the Study:
- To evaluate nucleic acid amplification testing (NAAT) as an alternative diagnostic tool for STIs in sexually abused children.
- To highlight the benefits of NAAT in pediatric forensic evaluations.
Main Methods:
- Review of NAAT as a diagnostic methodology for STIs.
- Comparison of NAAT sensitivity and specificity with traditional culture methods.
- Assessment of NAAT invasiveness in pediatric populations.
Main Results:
- Nucleic acid amplification testing demonstrates high sensitivity and specificity for identifying STIs.
- NAAT is less invasive than culture-based methods.
- NAAT presents a potentially more efficacious diagnostic option for children.
Conclusions:
- NAAT is a highly sensitive and specific method for STI detection in children.
- The less invasive nature of NAAT makes it a suitable alternative to cultures for sexually abused children.
- Implementing NAAT can improve the diagnostic experience for pediatric sexual abuse victims.

