Herpesvirus papio: state of viral DNA in baboon lymphoblastoid cell lines

Baboon lymphoblastoid cell lines (LCL) transformed with herpesvirus papio were evaluated for the state of HVP DNA in the cells by caesium chloride density-gradient centrifugation and DNA-cRNA filter hybridization. Five HVP producer cultures and one non-producer culture were studied and non-integrated and integrated HVP DNA were detected in all producer cultures whereas only integrated HVP DNA could be demonstrated in the non-producer LCL. Non-integrated HVP dna had a density of 1.715--1.716 g/cm3 in caesium chloride corresponding to a guanine + cytosine content of 56--57%.