Identification and partial purification of two EBV-associated DNA polymerases

Virally induced DNA polymerases have been demonstrated in cells infected with a variety of herpesviruses. These include herpes simplex virus (Weissbach et al., 1973), Marek's disease virus (Boezi et al., 1974), equine herpesvirus (Allen et al., 1977), and cytomegalovirus (Huang, 1975a). Recently a new iododeoxyuridine (IUDR)-induced intracellular DNA polymerase in an Epstein-Barr virus (EBV)-producing hybrid cell line has also been reported (Miller et al., 1977). We present evidence that there are two different DNA polymerase activities associated with EBV, one intracellular and the other virion-associated. Both of these enzymes have certain biochemical characteristics which distinguish them from each other, and from the host-cell DNA polymerases found in lymphocytes.