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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Related Experiment Video

Updated: May 27, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Single cell Raman spectroscopy for cell sorting and imaging.

Mengqiu Li1, Jian Xu, Maria Romero-Gonzalez

  • 1Kroto Research Institute, University of Sheffield, Sheffield S3 7HQ, UK.

Current Opinion in Biotechnology
|December 6, 2011
PubMed
Summary
This summary is machine-generated.

Single cell Raman spectroscopy (SCRS) offers label-free cellular analysis. This review explores methods to enhance weak Raman signals for improved biotechnological applications like cell sorting and imaging.

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Related Experiment Videos

Last Updated: May 27, 2026

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects
07:37

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
13:48

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Area of Science:

  • Biotechnology
  • Spectroscopy
  • Cell Biology

Background:

  • Single cell Raman spectroscopy (SCRS) is a non-invasive, label-free technique for analyzing individual living cells.
  • SCRS provides rich molecular information, acting as a unique fingerprint for cellular identification.
  • Weak spontaneous Raman signals are a major limitation of SCRS.

Purpose of the Study:

  • To review recent advancements in enhancing spontaneous Raman signals.
  • To discuss the biotechnological developments and applications of SCRS.

Main Methods:

  • Review of signal enhancement techniques: Resonance Raman Spectroscopy (RRS), Coherent Anti-Stokes Raman Spectroscopy (CARS), Stimulated Raman Spectroscopy (SRS), and Surface-Enhanced Raman Scattering (SERS).

Main Results:

  • These enhanced techniques significantly improve signal strength for SCRS.
  • Improved SCRS enables detailed analysis of cellular components like nucleic acids, proteins, carbohydrates, and lipids.

Conclusions:

  • Enhanced SCRS offers powerful capabilities for cellular analysis.
  • Applications include Raman Activated Cell Sorting (RACS) and advanced Raman imaging and mapping.