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Surface glycoprotein patterns of human haematopoietic cell lines
IARC Scientific Publications
|January 1, 1978
Summary
Human cells infected with Epstein-Barr virus (EBV) exhibit distinct surface glycoprotein patterns compared to uninfected cells. This difference, identified using a specific labeling method, aids in distinguishing EBV-positive from EBV-negative cell lines.
Area of Science:
- Cell biology
- Virology
- Biochemistry
Background:
- Epstein-Barr virus (EBV) is a human herpesvirus known to infect various hematopoietic cells.
- Understanding the molecular differences between EBV-infected and uninfected cells is crucial for diagnostics and therapeutic strategies.
Purpose of the Study:
- To investigate and differentiate surface glycoprotein profiles of human EBV-positive and EBV-negative hematopoietic cell lines.
- To establish a method for easily distinguishing between these two cell types based on their surface characteristics.
Main Methods:
- Utilized surface labeling techniques involving galactose oxidase and tritiated sodium borohydride.
- Analyzed and compared the resulting surface glycoprotein patterns of EBV-infected and EBV-uninfected hematopoietic cell lines.
Main Results:
- Demonstrated clearly distinguishable surface glycoprotein patterns between human EBV-positive and EBV-negative hematopoietic cell lines.
- The galactose oxidase-tritiated sodium borohydride labeling method proved effective in differentiating these cell populations.
Conclusions:
- Surface glycoprotein expression differs significantly between EBV-infected and EBV-uninfected human hematopoietic cells.
- This method provides a reliable approach for identifying EBV presence in hematopoietic cell lines.