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Updated: May 26, 2026
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Published on: May 7, 2018
Development of the first sphingomyelin biomimetic stationary phase for immobilized artificial membrane (IAM)
Dieter Verzele1, Frédéric Lynen, Mike De Vrieze
1Pfizer Analytical Research Centre, Department of Organic Chemistry, Ghent University, Krijgslaan 281-S4bis, B-9000 Ghent, Belgium.
Abstract:
A prototype sphingomyelin stationary phase for Immobilized Artificial Membrane (IAM) chromatography was synthesized by an ultra-short, solid-phase inspired methodology, in which an oxidative release monitoring strategy played a vital role. Evaluated in a proof-of-concept model for blood-brain barrier passage, partial least squares regression demonstrated its potential as an in vitro prediction tool.
