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Related Concept Videos

Karyotyping01:17

Karyotyping

Overview
Karyotyping01:17

Karyotyping

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Overview of Fungi01:29

Overview of Fungi

Fungi are a diverse group of eukaryotes more closely related to animals than other eukaryotes. Fungal cell walls comprise chitin, a polysaccharide that provides structural strength, and glucans, which contribute to flexibility and integrity. Other polysaccharides, such as mannans and galactosans, may supplement or replace chitin in some fungi. These adaptations, along with their preference for acidic environments and tolerance for high osmotic pressure, enable fungi to thrive in various...

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Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells
07:58

Use of Image Cytometry for Quantification of Pathogenic Fungi in Association with Host Cells

Published on: June 19, 2013

Karyotyping methods for fungi.

Rahim Mehrabi1, Masatoki Taga, Mostafa Aghaee

  • 1Seed and Plant Improvement Institute, Karaj, Iran. Rahim.Mehrabi@spii.ir

Methods in Molecular Biology (Clifton, N.J.)
|December 21, 2011
PubMed
Summary
This summary is machine-generated.

Pulsed field gel electrophoresis and germ tube burst methods separate fungal chromosomes for detailed analysis. These techniques are valuable for understanding fungal genetics, including chromosome size, number, and gene locations.

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Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Fungal chromosome analysis is crucial for understanding fungal biology and evolution.
  • Existing methods for fungal karyotyping have limitations in resolving large chromosomes or determining chromosome number accurately.

Purpose of the Study:

  • To provide detailed protocols for two complementary methods: pulsed field gel electrophoresis (PFGE) and the germ tube burst (GTB) method.
  • To highlight the applications of these methods in fungal biology, including chromosome polymorphism and gene mapping.

Main Methods:

  • Pulsed field gel electrophoresis (PFGE) for separating large fungal chromosomes (megabase size).
  • Germ tube burst (GTB) method for separating fungal chromosomes of any size, enabling chromosome number determination and in situ hybridization.

Main Results:

  • PFGE allows for the separation and analysis of fungal chromosomes up to several megabases.
  • GTB method effectively separates fungal chromosomes for accurate chromosome number determination and in situ hybridization.

Conclusions:

  • PFGE and GTB are complementary and valuable tools for fungal karyotyping and genetic analysis.
  • These detailed protocols facilitate research in fungal chromosome size and number polymorphisms, and gene localization.