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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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Updated: May 26, 2026

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

Surface enhanced Raman scattering for multiplexed detection.

Jennifer A Dougan1, Karen Faulds

  • 1Centre for Nanometrology, Department of Pure and Applied Chemistry, WestCHEM, University of Strathclyde, 295 Cathedral Street, Glasgow, G1 1XL, UK.

The Analyst
|December 22, 2011
PubMed
Summary
This summary is machine-generated.

Surface-enhanced Raman scattering (SERS) spectroscopy enables multiplexed detection of various biological targets in complex samples. This technique shows promise for both in vivo and in vitro disease detection and management.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Spectroscopy

Background:

  • Multiplexed detection of biological analytes is vital for disease management.
  • Surface-enhanced Raman scattering (SERS) spectroscopy is an emerging analytical technique.
  • SERS offers high sensitivity and specificity for detecting multiple targets simultaneously.

Purpose of the Study:

  • To review recent advancements in SERS spectroscopy for multiplexed biological detection.
  • To highlight the application of SERS in complex biological media.
  • To discuss the progression of SERS from dye molecules to complex biomolecules.

Main Methods:

  • Review of recent scientific literature on SERS applications in biological detection.
  • Analysis of SERS performance in complex matrices.
  • Evaluation of SERS for detecting DNA, proteins, and other biomolecules.

Main Results:

  • SERS has advanced from detecting simple dyes to complex biomolecules like DNA and proteins.
  • SERS allows for multiplexed detection in increasingly complex biological samples.
  • Recent studies demonstrate SERS multiplexing capabilities for both in vivo and in vitro applications.

Conclusions:

  • SERS spectroscopy is a powerful tool for multiplexed biological detection.
  • The technique is adaptable for diverse applications in diagnostics and research.
  • SERS holds significant potential for future advancements in intelligent disease management.