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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

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Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
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SAGA function in tissue-specific gene expression.

Vikki M Weake1, Jerry L Workman

  • 1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.

Trends in Cell Biology
|December 27, 2011
PubMed
Summary
This summary is machine-generated.

The Spt-Ada-Gcn5-acetyltransferase (SAGA) complex

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Area of Science:

  • Molecular Biology
  • Genetics

Background:

  • The Spt-Ada-Gcn5-acetyltransferase (SAGA) complex is a crucial transcription coactivator.
  • SAGA possesses functionally distinct modules, enabling diverse regulatory roles in transcription.
  • Its ubiquitin protease activity has been implicated in regulating gene expression.

Purpose of the Study:

  • To explore the role of SAGA's ubiquitin protease activity in tissue-specific gene expression in Drosophila.
  • To elucidate the mechanisms by which SAGA is recruited to target promoters.
  • To propose a model for how SAGA-mediated histone deubiquitination regulates gene activation.

Main Methods:

  • Analysis of SAGA's modular structure and recruitment mechanisms.
  • Investigating the function of ubiquitin protease activity in gene regulation.
  • Hypothesizing the impact of histone H2B deubiquitination on gene expression.

Main Results:

  • Identified a role for SAGA's ubiquitin protease activity in Drosophila tissue-specific gene expression.
  • Proposed that genes requiring SAGA's ubiquitin protease activity depend on histone H2B deubiquitination for activation.
  • Hypothesized that deubiquitination destabilizes promoter nucleosomes, enhancing RNA polymerase II recruitment.

Conclusions:

  • SAGA's ubiquitin protease activity is vital for specific gene expression patterns.
  • Deubiquitination of histone H2B by SAGA promotes transcription by destabilizing nucleosomes.
  • This process may also aid in transitioning paused RNA polymerase II into elongation.