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Updated: May 26, 2026

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts
06:32

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Published on: April 13, 2022

Proline metabolism and cancer.

James M Phang1, Wei Liu

  • 1Metabolism and Cancer Susceptibility Section, Laboratory of Comparative Carcinogenesis, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA. phangj@mail.nih.gov

Frontiers in Bioscience (Landmark Edition)
|December 29, 2011
PubMed
Summary

Proline oxidase (POX) induces cancer cell death via ROS production. Targeting miR-23b* to restore POX offers a novel cancer therapy strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Proline metabolism is crucial in cancer.
  • Proline oxidase (POX) is regulated by the tumor suppressor P53.
  • POX can induce apoptosis and affect tumor growth.

Purpose of the Study:

  • Investigate the role of POX in cancer metabolism and its therapeutic potential.
  • Identify mechanisms regulating POX expression in tumors.
  • Explore miR-23b* as a therapeutic target for cancer.

Main Methods:

  • Ectopic POX expression using tet-off system.
  • Assessment of reactive oxygen species (ROS) production.
  • Xenograft tumor growth studies in immunodeficient mice.
  • Analysis of POX expression in human tumors.

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  • MicroRNA (miRNA) expression profiling and functional studies.
  • Antagomir treatment to inhibit miR-23b*.

    • Main Results:

    • Ectopic POX expression triggered mitochondrial apoptosis via ROS.
    • POX overexpression suppressed xenograft tumor growth.
    • POX levels were decreased in digestive tract and kidney tumors.
    • Downregulation was not due to gene mutations or hypermethylation.
    • High miR-23b* levels in tumors inhibited POX expression.
    • Antagomirs of miR-23b* restored POX expression and tumor-suppressive effects.

    Conclusions:

    • POX exhibits tumor-suppressive functions modulated by miR-23b*.
    • Restoring POX expression via miR-23b* inhibition is a potential cancer therapeutic strategy.
    • POX's role in cancer is complex, mediating both cell death and survival depending on context.