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[The functional characteristics of the peripheral blood granulocytes in erysipelatous inflammation]

O I Krifuks, I G Tsoĭ, G E Sagimbaeva

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |June 1, 1990
    PubMed

    Insights

    In erysipelas patients, polymorphonuclear leukocytes (PML) show altered receptor expression and function. "Light" PML are linked to leukocyte activation by inflammation, not bone marrow release.

    Area of Science:

    • Immunology
    • Hematology

    Background:

    • Erysipelas involves bacterial infection affecting the skin.
    • Polymorphonuclear leukocytes (PML) play a crucial role in the innate immune response to bacterial infections.

    Purpose of the Study:

    • To investigate the characteristics of low-density (light) and normal-density PML in erysipelas patients.
    • To compare the functional activity and receptor expression of PML in erysipelas patients versus healthy donors.

    Main Methods:

    • Isolation of PML from erysipelas patients and healthy donors using Ficoll-Verographin density gradients.
    • Assessment of Fc receptor (FcR) and C3 receptor (C3R) expression.
    • Evaluation of functional activity using the nitroblue tetrazolium (NBT) test.

    Main Results:

    • No significant difference in average indices between light and normal PML in erysipelas patients.
    • Erysipelas patients' PML exhibited higher C3R expression, spontaneous NBT positivity, and reduced response to IgG stimulation compared to donors.
    • Treatment of erysipelas patient blood with Streptococcus haemolyticus allergen increased light PML, while brucellin had no effect.

    Conclusions:

    • The presence of light PML in erysipelas is associated with leukocyte activation by inflammatory products, not increased release from bone marrow.
    • These findings highlight altered PML function and density in erysipelas, suggesting a role in disease pathogenesis.

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