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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
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Live-cell dSTORM of cellular DNA based on direct DNA labeling

Alexander Benke1, Suliana Manley

  • 1Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, 1005 Lausanne, Switzerland.

Chembiochem : a European Journal of Chemical Biology
|January 4, 2012
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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