Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
Structure-Activity Relationships and Drug Design01:28

Structure-Activity Relationships and Drug Design

Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence its...
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
Dose-Response Relationship: Selectivity and Specificity01:25

Dose-Response Relationship: Selectivity and Specificity

Drugs exert their therapeutic effects by interacting with receptors, enzymes, or ion channels that are present throughout the human body. The strength and duration of the interaction between a drug and its target receptor are characterized by the selectivity and specificity of the drug. Selectivity refers to a drug's strong preference for its intended target over other targets. For instance, isoprenaline, a non-selective β-adrenergic agonist, interacts with both β1- and β2-adrenergic receptors...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Astrocytes viewed through the lens of their proteomes and subproteomes.

Nature reviews. Neuroscience·2026
Same author

<i>Arabidopsis</i> YEATS domain proteins facilitate DNA double-strand break repair via homology-directed pathways.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Identification of a Putative Metal Transporter in the Apicoplast of Malaria Parasites.

bioRxiv : the preprint server for biology·2026
Same author

The Pseudomonas aeruginosa ribonuclease Ribocin cleaves eukaryotic ribosomes at helix 69 to inhibit host translation.

PLoS biology·2026
Same author

Drp1 regulates mitochondrial health and controls skeletal muscle mass through the Erk1/2-Nur77 pathway.

Science advances·2026
Same author

Acyl Carrier Protein is Essential for Apicoplast Biogenesis in Malaria Parasites Independent of Fatty Acid Synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Methods to Validate Binding and Kinetics of "Proximity-Inducing" Covalent Immune-Recruiting Molecules.

Current protocols in chemical biology·2020
Same journal

Multiparametric High-Content Assays to Measure Cell Health and Oxidative Damage as a Model for Drug-Induced Liver Injury.

Current protocols in chemical biology·2020
Same journal

Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting.

Current protocols in chemical biology·2020
Same journal

Three-Color Imaging Enables Simultaneous Screening of Multiple RNA Targets on Small Molecule Microarrays.

Current protocols in chemical biology·2020
Same journal

Azide-Terminated RAFT Polymers for Biological Applications.

Current protocols in chemical biology·2020
Same journal

Discovery of Electrophiles and Profiling of Enzyme Cofactors.

Current protocols in chemical biology·2020
See all related articles

Related Experiment Video

Updated: May 26, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

Target identification using drug affinity responsive target stability (DARTS).

Brett Lomenick1, Gwanghyun Jung, James A Wohlschlegel

  • 1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles.

Current Protocols in Chemical Biology
|January 10, 2012
PubMed
Summary
This summary is machine-generated.

Drug Affinity Responsive Target Stability (DARTS) identifies protein-ligand interactions by detecting target stabilization. This method works with complex mixtures, aiding in target identification and affinity estimation.

More Related Videos

Protein Target Prediction and Validation of Small Molecule Compound
10:21

Protein Target Prediction and Validation of Small Molecule Compound

Published on: February 23, 2024

Related Experiment Videos

Last Updated: May 26, 2026

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction
05:28

A Semi-Quantitative Drug Affinity Responsive Target Stability (DARTS) assay for studying Rapamycin/mTOR interaction

Published on: August 27, 2019

Protein Target Prediction and Validation of Small Molecule Compound
10:21

Protein Target Prediction and Validation of Small Molecule Compound

Published on: February 23, 2024

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Proteomics

Background:

  • Understanding protein-ligand interactions is crucial in drug discovery.
  • Existing methods for target identification can be complex and require purified proteins.

Purpose of the Study:

  • To introduce and describe the Drug Affinity Responsive Target Stability (DARTS) methodology.
  • To highlight DARTS's utility in identifying and studying protein-ligand interactions.

Main Methods:

  • DARTS relies on the principle that ligand binding stabilizes target proteins against protease degradation.
  • The technique can be applied to crude cell lysates and complex protein mixtures, avoiding the need for purified proteins.
  • Native, unmodified small molecules are used in the assay.

Main Results:

  • DARTS enables the identification of protein targets for small molecules.
  • The method can validate predicted or previously identified protein-ligand interactions.
  • DARTS can be used to estimate the binding affinity between proteins and small molecules.

Conclusions:

  • DARTS is a versatile and accessible methodology for studying protein-ligand interactions.
  • Its simplicity and applicability to complex mixtures make it valuable for initial target identification and validation.
  • The described protocols can be adapted for various research needs.