Related Experiment Video
Updated: May 26, 2026
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
Published on: April 8, 2012
[Construction of eukaryotic expression vector and package of lentivirus vector encoding human leukocyte antigen-G5]
Xin-yu Guo1, Feng Jiang, Hong-xi Zhao
1Department of Gynecology and Obstetrics, the Chniese PLA General Hospital, Beijing 100853, China.
Aim:
To construct a lentiviral expression vector carrying HLA-G5.
Methods:
The CDs region of HLA-G5 gene was cloned into the lentiviral vector by restriction endonuclease Nhe I/Not I digestion and T4DNA ligase ligation. After transformation into completent E.coli cells, the candidate clones were identified by PCR and kidney cell line 293T cells by lipofectamine 2000 to produce the lentiviral particles, and the viral titer was determined.
Results:
The lentiviral vector pCDH-CMV-MCS-EF1-copGFP Vector for HLA-G5 was constructed successfully, and the virus in the supernatant reached a titer of TU/mL.
Conclusion:
This research completed the package of lentivirus vector encoding HLA-G5 as a tool for further study.
