Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Comparison and optimization of total ionic strength adjustment buffer during detecting fluoride in trace serum sample by fluoride ion selective electrode method].

Wei sheng yan jiu = Journal of hygiene research·2016
Same author

Flexible Transparent Electronic Gas Sensors.

Small (Weinheim an der Bergstrasse, Germany)·2016
Same author

Magnetoresistance in Co/2D MoS2/Co and Ni/2D MoS2/Ni junctions.

Physical chemistry chemical physics : PCCP·2016
Same author

Development and Validation of an Interactive Efficient Dose Rates Distribution Calculation Program Arshield for Visualization of Radiation Field in Nuclear Power Plants.

Radiation protection dosimetry·2016
Same author

[Effects of land use change on landscape pattern vulnerability in Yinchuan Basin, Northwest China].

Ying yong sheng tai xue bao = The journal of applied ecology·2016
Same author

PreSurgMapp: a MATLAB Toolbox for Presurgical Mapping of Eloquent Functional Areas Based on Task-Related and Resting-State Functional MRI.

Neuroinformatics·2016

Related Experiment Video

Updated: May 26, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Stable integration of an engineered megabase repeat array into the maize genome.

Han Zhang1, Bao H Phan, Kai Wang

  • 1Department of Genetics, University of Georgia, Athens, GA 30602, USA.

The Plant Journal : for Cell and Molecular Biology
|January 12, 2012
PubMed
Summary

Synthetic DNA arrays were engineered and delivered directly into maize genomes. This breakthrough enables the stable introduction of large DNA segments, advancing plant genome engineering and artificial chromosome design.

More Related Videos

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Related Experiment Videos

Last Updated: May 26, 2026

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
10:28

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

Published on: February 14, 2020

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Area of Science:

  • Plant Biology
  • Genetics
  • Molecular Biology

Background:

  • Stable introduction of large DNA segments is crucial for plant genome engineering.
  • Current methods face limitations in delivering complex DNA sequences effectively.

Purpose of the Study:

  • To engineer and introduce centromere-sized satellite repeat arrays into maize.
  • To demonstrate the feasibility of delivering synthetic DNA constructs directly into plant cells.

Main Methods:

  • Designed a synthetic 156 bp repeat monomer with five DNA-binding motifs.
  • Extended monomers into tandem arrays using overlapping PCR.
  • Transformed synthetic arrays directly into maize via biolistic transformation.

Main Results:

  • Successfully introduced synthetic repeat arrays into maize, with insertions at three loci.
  • The longest array achieved a size of at least 1100 kb.
  • Demonstrated efficient tethering of YFP to arrays using the LacI DNA-binding module.

Conclusions:

  • Synthetic repeats can be delivered directly into plant cells, bypassing bacterial propagation.
  • These synthetic arrays generally insert into a single locus, facilitating targeted genome modification.
  • The methodology supports the development of artificial chromosomes and advanced plant genome engineering.