Related Experiment Video
Updated: May 25, 2026
Vitrification of Ovarian Cortex Tissue to Achieve a Glassy State of Aggregation
Published on: August 9, 2024
Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000
Xiuwen Tan1, Enliang Song, Xiaomu Liu
1Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Husbandry and Veterinary Medicine, Shandong Academy of Agriculture Sciences, Jinan, Shandong, China.
Abstract:
The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5% ethylene glycol (EG) + 12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG + 10% DMSO or 15% EG + 15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.
