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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 25, 2026

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles
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Multinode acoustic focusing for parallel flow cytometry.

Menake E Piyasena1, Pearlson P Austin Suthanthiraraj, Robert W Applegate

  • 1Center for Biomedical Engineering, Department of Chemical and Nuclear Engineering, The University of New Mexico, Albuquerque, New Mexico 87131-0001, USA.

Analytical Chemistry
|January 14, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces novel multinode acoustic focusing flow cells to overcome limitations in conventional flow cytometry. These cells enable high-throughput analysis of larger particles and increased cell analysis rates, advancing rare cell detection and large-volume applications.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Conventional flow cytometry has limitations in analyzing particles larger than 70 micrometers, at high flow rates, or exceeding 50,000 analyses per second.
  • These limitations hinder applications such as rare cell detection and large-volume sample processing.

Purpose of the Study:

  • To develop advanced flow cells overcoming conventional flow cytometry limitations.
  • To enable high-throughput analysis of a wider range of particle sizes and at increased rates.

Main Methods:

  • Development of multinode acoustic focusing flow cells.
  • Positioning particles, from red blood cells to 107 micrometers, into up to 37 parallel flow streams.
  • Demonstration using alignment microspheres, red blood cells, and CD4+ immunophenotyping assays.

Main Results:

  • Successfully focused particles ranging from red blood cells to 107 micrometers in diameter.
  • Demonstrated parallel flow stream capability for high-throughput analysis.
  • Validated the system with microspheres, red blood cells, and a CD4+ assay.

Conclusions:

  • Multinode acoustic focusing flow cells significantly enhance flow cytometry capabilities.
  • This technology enables high-throughput analysis for rare cell detection, large particle analysis, and high-volume applications.
  • The developed flow cells represent a significant advancement for future flow cytometry instrumentation.