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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

A label-free cell separation using surface acoustic waves.

Myeong Chan Jo1, Rasim Guldiken

  • 1Department of Mechanical Engineering, University of South Florida, Tampa, FL 33620, USA. mjo@mail.usf.edu

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|January 19, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a two-stage microfluidic device for label-free cell separation using surface acoustic waves. The platform efficiently separates cells by size without external sheath flow.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Acoustics

Background:

  • Cell separation is crucial in various biological and medical applications.
  • Traditional methods often require cell labeling or complex procedures.
  • Microfluidic devices offer miniaturized and precise cell manipulation.

Purpose of the Study:

  • To develop a continuous, label-free cell separation method.
  • To utilize surface acoustic waves (SAW) for cell focusing and separation.
  • To eliminate the need for sheath flow in microfluidic cell sorting.

Main Methods:

  • A two-stage microfluidic platform was designed and fabricated.
  • Standing surface acoustic waves were employed for initial cell focusing.
  • Acoustic force differences were used for size-dependent cell separation.
  • Demonstrated separation of 3 μm and 10 μm particles.

Main Results:

  • Cells were successfully lined up and focused at the channel center using SAW.
  • Different sized particles exhibited distinct lateral displacements based on acoustic forces.
  • Simultaneous focusing and separation were achieved in a single device.
  • Effective separation of particle streams without sheath flow was demonstrated.

Conclusions:

  • The proposed two-stage microfluidic device enables efficient, label-free cell separation.
  • Surface acoustic waves are a viable tool for precise cell manipulation in microfluidics.
  • This technology offers a simplified and sheath-flow-free approach for cell sorting.