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Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
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Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
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Nucleic Acids and Nucleotides01:20

Nucleic Acids and Nucleotides

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Characteristics and Nomenclature of Copolymers

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Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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Published on: January 19, 2019

Poly(alkylene oxide) copolymers for nucleic acid delivery.

Swati Mishra1, Lavanya Y Peddada, David I Devore

  • 1Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, 08854, United States.

Accounts of Chemical Research
|January 21, 2012
PubMed
Summary

Polymer and lipid nanoassemblies, particularly poly(alkylene oxide) (PAO) copolymers, are advancing gene delivery for therapeutics. Optimizing hydrophobic-lipophilic balance (HLB) enhances serum stability and cellular uptake for improved gene therapy efficacy.

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Last Updated: May 25, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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Published on: January 19, 2019

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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Background:

  • Advancing gene-based therapeutics to the clinic is hindered by challenges in safe and effective nucleic acid delivery to target tissues.
  • Polymer and lipid-based nanoassemblies have shown promise in preclinical and early clinical studies for various diseases.
  • Current research focuses on developing biocompatible, stable, and targeted gene delivery systems to enhance therapeutic outcomes.

Purpose of the Study:

  • To review the use of poly(alkylene oxide) (PAO) polymers, including Pluronic copolymers, in nucleic acid delivery systems.
  • To highlight the critical role of hydrophobic-lipophilic balance (HLB) in designing effective gene delivery vehicles.
  • To discuss innovative modifications of PAO-based carriers for improved gene delivery.

Main Methods:

  • Review of polycation-PAO conjugates, liposome-PAO formulations, and PAO micelles for nucleic acid delivery.
  • Analysis of polymer design considerations, including molecular weight and block length, with a focus on HLB.
  • Discussion of macromolecular modifications such as PAO graft copolymers, PAO gel-based carriers, and biodegradable PAO amphiphilic block copolymers.

Main Results:

  • Poly(alkylene oxide) (PAO) polymers, especially Pluronic triblock copolymers, are versatile for nucleic acid delivery.
  • Hydrophobic-lipophilic balance (HLB) is a critical parameter influencing serum stability and cell membrane association of gene delivery systems.
  • PAO-based modifications enhance serum stability, cellular uptake, and enable sustained or stimuli-responsive delivery.

Conclusions:

  • PAO-based nanoassemblies offer significant potential for improving gene therapy efficacy and safety.
  • Strategic manipulation of polymer architecture, particularly HLB, is key to optimizing gene delivery performance.
  • Continued innovation in PAO-based materials promises more effective and targeted nucleic acid therapeutics.