An artery-specific fluorescent dye for studying neurovascular coupling
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Updated: May 25, 2026
Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo
Published on: November 20, 2021
Zhiming Shen1, Zhongyang Lu, Pratik Y Chhatbar
1Department of Neurosciences, Medical University of South Carolina, Charleston, South Carolina, USA.
Abstract:
We demonstrate that Alexa Fluor 633 hydrazide (Alexa Fluor 633) selectively labels neocortical arteries and arterioles by binding to elastin fibers. We measured sensory stimulus-evoked arteriole dilation dynamics in mouse, rat and cat visual cortex using Alexa Fluor 633 together with neuronal activity using calcium indicators or blood flow using fluorescein dextran. Arteriole dilation decreased fluorescence recorded from immediately underlying neurons, representing a potential artifact during neuronal functional imaging experiments.
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