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Updated: May 25, 2026
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Comparison of the methods for seeding human bone marrow mesenchymal stem cells to macroporous alginate cryogel
Yu A Petrenko1, R V Ivanov, V I Lozinsky
1Institute of Cryobiology and Cryomedicine Problems, National Academy of Sciences of Ukraine, Khar'kov. yu.a.petrenko@gmail.com
Abstract:
We performed a comparative study of the localization, distribution, metabolic activity, and surface properties of human bone marrow mesenchymal stromal cells after static and perfusion seeding to macroporous alginate cryogels. A simple perfusion system for mesenchymal stromal cell seeding to macroporous alginate cryogel sponges proposed in this study resulted in rapid and uniform distribution of cells within the whole volume of the scaffold preserving functional and morphological properties of the cells.
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