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Related Experiment Videos

Reduced membrane fluidity in platelets from diabetic patients.

P D Winocour1, M Bryszewska, C Watala

  • 1Department of Pathology, McMaster University, Hamilton, Ontario, Canada.

Diabetes
|February 1, 1990
PubMed
Summary

Diabetic platelets show reduced membrane fluidity, potentially linked to altered lipid profiles. This may contribute to hypersensitivity to agonists like thrombin, impacting platelet function in diabetes.

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Area of Science:

  • Biochemistry
  • Hematology
  • Metabolic Disorders

Background:

  • Platelets from diabetic patients exhibit hypersensitivity to agonists in vitro.
  • Reduced platelet membrane fluidity, associated with cholesterol enrichment, is linked to this hypersensitivity.
  • An increased cholesterol-phospholipid molar ratio in platelet membranes is hypothesized to cause decreased fluidity.

Purpose of the Study:

  • To investigate platelet response to thrombin in diabetic subjects.
  • To assess platelet membrane fluidity in diabetic individuals.
  • To determine the platelet cholesterol-phospholipid molar ratio in diabetes.

Main Methods:

  • Compared 12 poorly controlled diabetic subjects with 12 age- and sex-matched controls.
  • Measured [14C]serotonin release from washed platelets in response to thrombin.

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  • Assessed platelet membrane fluidity using 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence polarization.
  • Analyzed plasma lipoprotein cholesterol concentrations and platelet cholesterol-phospholipid molar ratio.

    • Main Results:

    • While mean serotonin release was not significantly different, 8/12 diabetic subjects showed a greater response to low-dose thrombin.
    • Mean fluorescence polarization was significantly greater in diabetic platelets, indicating decreased membrane fluidity.
    • Plasma VLDL + LDL to HDL2 + HDL3 ratio was significantly higher in diabetics, but total platelet cholesterol-phospholipid ratio did not differ.

    Conclusions:

    • Diabetic platelets exhibit decreased membrane fluidity.
    • This reduced fluidity may contribute to altered platelet function and hypersensitivity in diabetes.
    • Further research is needed to fully elucidate the role of lipid alterations in diabetic platelet pathophysiology.