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Updated: May 25, 2026
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Different expression of CD146 in human normal and osteosarcoma cell lines
Concetta Schiano1, Vincenzo Grimaldi, Amelia Casamassimi
1Department of General Pathology, Excellence Research Centre on Cardiovascular Disease, U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), 1st School of Medicine, Second University of Naples, Piazza Miraglia 2, 80138, Naples, Italy.
CD146, a cell adhesion molecule, is highly expressed in osteosarcoma (OS) cells compared to normal cells. Silencing the Yin Yang 1 transcription factor reduced CD146 overexpression in these tumor cells.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- CD146 is a cell membrane adhesion molecule implicated in tumor progression and metastasis.
- High CD146 expression correlates with increased metastatic potential in various cancers.
Purpose of the Study:
- To investigate CD146 expression in human osteosarcoma (OS) cell lines.
- To analyze the relationship between CD146 and the transcription factor Yin Yang 1 (YY1) in OS.
Main Methods:
- Examined CD146 protein and RNA levels in normal and OS osteoblast-like cell lines.
- Utilized multiple methods for expression analysis.
- Investigated CD146 levels in shYY1 cells where YY1 was silenced.
Main Results:
- CD146 protein is lowly expressed in normal osteoblasts but highly expressed in all analyzed OS cell lines (SaOS, MG-63, U-2OS).
- CD146 overexpression was partially reduced in OS cells with silenced YY1.
- YY1 transcription factor is also overexpressed in human OS cells.
Conclusions:
- CD146 is significantly upregulated in human osteosarcoma.
- The transcription factor YY1 may play a role in regulating CD146 overexpression in osteosarcoma.
