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Updated: May 25, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
Published on: April 9, 2014
Improved signal-to-noise ratio in laboratory-based phase contrast tomography
Matthieu N Boone1, Yoni De Witte, Manuel Dierick
1Department of Physics and Astronomy, Ghent University, Proeftuinstraat 86, B-9000 Gent, Belgium. Matthieu.Boone@UGent.be
Abstract:
In conventional X-ray microtomography (μCT), the three-dimensional (3D) distribution of the attenuation coefficient of X-rays is measured and reconstructed in a 3D volume. As spatial resolution increases, the refraction of X-rays becomes a significant phenomenon in the imaging process. Although this so-called phase contrast was initially a cumbersome feature in lab-based μCT, special phase retrieval algorithms were developed to exploit these effects. Clear advantages in terms of visualization and analysis can be seen when phase retrieval algorithms are applied, including an increased signal-to-noise ratio. In this work, this is demonstrated both on simulated and measured data.
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