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Updated: May 25, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

A CMOS Time-Resolved Fluorescence Lifetime Analysis Micro-System.

Bruce R Rae¹, Keith R Muir, Zheng Gong

¹Institute for Integrated Micro and Nano Systems, The School of Engineering, The University of Edinburgh, The King's Buildings, Mayfield Road, Edinburgh, EH9 3JL, UK; E-Mails: Keith.Muir@ed.ac.uk (K.R.M.); Robert.Henderson@ed.ac.uk (R.K.H.); David.Renshaw@ee.ed.ac.uk (D.R.).

Sensors (Basel, Switzerland)

|February 1, 2012

Summary

This summary is machine-generated.

This study introduces a compact CMOS micro-system for fluorescence lifetime analysis, replacing bulky equipment. The system enables precise measurements using micro-LEDs and single-photon avalanche diodes.

Keywords:

CMOS GaN fluorescence lifetime micro light-emitting diodes (micro-LEDs)micro-system single-photon avalanche diodes

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Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

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Published on: January 18, 2017

Area of Science:

Photonics and Instrumentation
Microelectronic Systems
Materials Science

Background:

Traditional fluorescence lifetime analysis relies on complex, bulky instrumentation.
Integrating advanced photonic and electronic components is crucial for miniaturization.

Purpose of the Study:

To develop a compact, CMOS-based micro-system for time-resolved fluorescence lifetime analysis.
To demonstrate the system's capability in analyzing fluorescent samples.

Main Methods:

Fabrication of a 16x4 array of single-photon avalanche diodes (SPADs) in high-voltage CMOS.
Integration of an 8x8 blue micro-LED array with LED drivers in low-voltage CMOS.
Development of in-pixel time-gated photon counting circuitry.
Utilizing a PC-based system to replace conventional instrumentation.

Main Results:

Successful fabrication of integrated SPAD and micro-LED arrays on a single chip.
Generation of narrow excitation pulses (777 ps FWHM) using the micro-LED array.
Demonstration of time-resolved fluorescence lifetime measurements on colloidal quantum dots and Rhodamine samples.

Conclusions:

The developed CMOS micro-system offers a miniaturized and efficient alternative for fluorescence lifetime analysis.
This technology has the potential to simplify and enhance fluorescence-based research and diagnostics.