Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Testosterone: Functions and Regulation

Testosterone: Functions and Regulation

The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...

Disorders of the Male Reproductive System

Disorders of the Male Reproductive System

Men's health issues are increasingly recognized as significant, with several conditions posing common threats. Among these, testicular cancer is especially prevalent in younger men, particularly those aged 20 to 35 years. The disease often manifests as a painless mass in the testicles, sometimes accompanied by a sensation of heaviness or a dull ache.
Prostate disorders are another major concern. These conditions can impair urinary flow due to the prostate's location around the urethra. Symptoms...

Testes: Histology

Testes: Histology

A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...

Testes: Gross Anatomy

Testes: Gross Anatomy

The testes, also known as testicles, are the male gonads. They are housed within the scrotum, a sac-like structure located beneath the penis. The scrotum's primary role is to regulate the temperature of the testes, which is crucial for sperm production.
Each testis is surrounded by the tunica albuginea, a dense connective tissue layer that provides structural support and protection. This layer is covered by an outer serous membrane called the tunica vaginalis, which helps reduce friction...

Serum Studies: Renal Function Tests

Serum Studies: Renal Function Tests

Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.

Signs of Puberty

Signs of Puberty

Puberty is a critical phase, typically beginning between the ages of 8 and 13 in girls and 9 and 14 in boys, though timing can vary based on genetics, environmental factors, and overall health. This period is characterized by the development of secondary sexual characteristics and the attainment of reproductive potential. Endocrine changes underpin puberty, with hormonal surges of Luteinizing Hormone (LH) and Follicle-Stimulating Hormone (FSH) instigated by Gonadotropin-Releasing Hormone (GnRH)...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimization of hemolysis, icterus and lipemia interference thresholds for 35 clinical chemistry assays.

Practical laboratory medicine·2021

Same author

Association of Cord Blood Thyroid-Stimulating Hormone Levels with Maternal, Delivery and Infant Factors.

Annals of the Academy of Medicine, Singapore·2021

Same author

What are the appropriate reference limits for the diagnosis of hypophosphataemia in paediatric patients?

Journal of clinical pathology·2019

Same author

Commutable whole blood reference materials for hemoglobin A1c validated on multiple clinical analyzers.

Clinical chemistry and laboratory medicine·2018

Same author

Pragmatic and evidence-based approach to paediatric cerebrospinal fluid reference limits for white cell count and concentrations of total protein and glucose.

Journal of clinical pathology·2018

Same author

Gestational age-specific reference intervals for serum thyroid hormone levels in a multi-ethnic population.

Clinical chemistry and laboratory medicine·2017

Search research articles

Related Experiment Video

Updated: May 25, 2026

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

Published on: March 2, 2011

Testosterone testing in adult males.

Clement K M Ho¹

¹Division of Laboratory Medicine, Department of Pathology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. clementho@ummc.edu.my

The Malaysian Journal of Pathology

|February 4, 2012

Summary

This summary is machine-generated.

Testosterone testing in adult men is rising. This review covers testosterone

More Related Videos

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Published on: August 14, 2019

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

Published on: July 10, 2017

Related Experiment Videos

Last Updated: May 25, 2026

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

Published on: March 2, 2011

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

Published on: August 14, 2019

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

A Modified Trier Social Stress Test for Vulnerable Mexican American Adolescents

Published on: July 10, 2017

Area of Science:

Biochemistry and Physiology
Clinical Chemistry
Endocrinology

Background:

Increasing demand for testosterone testing in adult males.
Need for a comprehensive overview for chemical pathologists and clinical biochemists.

Purpose of the Study:

Outline male testosterone biochemistry and physiology.
Summarize laboratory methods for assessing testosterone status.
Discuss diagnostic criteria for late-onset hypogonadism (LOH) and other indications for testing.

Main Methods:

Review of existing literature on testosterone biochemistry, physiology, and laboratory assays.
Critical analysis of diagnostic criteria for LOH.
Exploration of varied indications for testosterone level assessment.

Main Results:

Detailed explanation of total testosterone, free testosterone, calculated free testosterone (CFT), bioavailable testosterone (BAT), and free androgen index (FAI) methodologies.
Discussion on the interpretation of laboratory results for diagnosing LOH.
Overview of other clinical scenarios necessitating testosterone testing, including male infertility.

Conclusions:

Provides essential biochemical and physiological context for testosterone testing.
Clarifies laboratory methodologies and diagnostic interpretation for LOH.
Offers a comprehensive guide to testosterone testing indications in adult men.