Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Polyester sulphonic Acid interstitial nanocomposite platform for peroxide biosensor.

Amir Al-Ahmed1, Peter M Ndangili, Nazeem Jahed

  • 1SensorLab, Department of Chemistry, University of Western Cape, Bellville, 7535, Cape Town, South Africa; E-Mails: aal-ahmed@uwc.ac.za (A.A.-A.); pndangili@uwc.ac.za (P.M.N.); njahed@uwc.ac.za (N.J.); eiwuoha@uwc.ac.za (E.I.I.).

Sensors (Basel, Switzerland)
|February 4, 2012
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioelectrochemical Sensing Dynamics of SARS-CoV-2 Biomarkers.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Recent trends in MOF-based electrochemical biosensing strategies for cardiovascular disease diagnostics.

Bioelectrochemistry (Amsterdam, Netherlands)·2026
Same author

Controlling the Optoelectronics of Poly(3-hexylthiophene-2,5-diyl) Dendritic Star Copolymers on a Polypropylenimine Core as Donor Materials for Organic Solar Cells.

ACS omega·2026
Same author

Electromimetic molecularly imprinted Polymersensor for wastewater emtricitabine.

Analytica chimica acta·2024
Same author

Terbium- and samarium-doped Li<sub>2</sub>ZrO<sub>3</sub> perovskite materials as efficient and stable electrocatalysts for alkaline hydrogen evolution reactions.

Environmental science and pollution research international·2024
Same author

3-Methyl Thiophene-Modified Boron-Doped Diamond (BDD) Electrodes as Efficient Catalysts for Phenol Detection-A Case Study for the Detection of Gallic Acid in Three Specific Tea Types.

Foods (Basel, Switzerland)·2024

A new biosensor platform using sulfonated polyaniline nanotubes immobilized horseradish peroxidase was developed for sensitive hydrogen peroxide detection. This novel enzyme immobilization method offers good stability and a low detection limit for H2O2.

Area of Science:

  • Electrochemistry
  • Nanomaterials
  • Biotechnology

Background:

  • Enzyme immobilization is crucial for biosensor development.
  • Developing stable and sensitive enzyme immobilization platforms is an ongoing challenge.
  • Polyaniline-based nanocomposites offer promising electrochemical properties.

Purpose of the Study:

  • To create a novel enzyme immobilization platform using sulfonated polyaniline (PANI) nanotubes.
  • To immobilize horseradish peroxidase (HRP) for hydrogen peroxide (H2O2) detection.
  • To characterize the electrochemical properties and performance of the developed biosensor.

Main Methods:

  • Polymerization of aniline within polyester sulphonic acid sodium salt (PESA) pores to form PANI nanotubes on a platinum electrode.
Keywords:
HRPelectrochemical polymerisationimmobilization of enzymeperoxide biosensorspolyaniline nanotubespolyester sulphonic acid

More Related Videos

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Related Experiment Videos

Last Updated: May 25, 2026

The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

  • Scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) for material characterization.
  • Electrochemical measurements using cyclic voltammetry and amperometry for H2O2 detection with immobilized HRP.

    • Main Results:

    • Homogeneous PANI nanotubes (∼90 nm) were successfully formed and stable up to 230 °C.
    • The PANI:PESA nanocomposite exhibited quasi-reversible redox behavior.
    • The HRP-immobilized biosensor demonstrated a sensitivity of 1.33 μA (μM)(-1) and a detection limit of 0.185 μM for H2O2.
    • The biosensor retained over 64% sensitivity after four days of storage.

    Conclusions:

    • A novel and stable enzyme immobilization platform was successfully developed.
    • The PANI:PESA nanocomposite is a suitable matrix for HRP immobilization.
    • The developed biosensor shows high sensitivity and stability for hydrogen peroxide detection.