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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 25, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Reconstruction procedure for 3D micro X-ray absorption fine structure.

Lars Lühl1, Ioanna Mantouvalou, Wolfgang Malzer

  • 1Bundesanstalt für Materialforschung und -prüfung, Berlin, Germany. lars.luehl@bam.de

Analytical Chemistry
|February 7, 2012
PubMed
Summary
This summary is machine-generated.

A novel 3D Micro-XAFS spectroscopy method combines X-ray Fluorescence and X-ray Absorption Fine Structure techniques. This approach enables depth-resolved chemical speciation in stratified materials without sample destruction.

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Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
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Published on: July 5, 2016

Related Experiment Videos

Last Updated: May 25, 2026

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
08:46

Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

Published on: April 13, 2016

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles
10:00

Energy Dispersive X-ray Tomography for 3D Elemental Mapping of Individual Nanoparticles

Published on: July 5, 2016

Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Chemical speciation in stratified materials is crucial for understanding material properties and behavior.
  • Conventional methods often struggle with depth-resolved analysis in complex layered structures.
  • X-ray Absorption Fine Structure (XAFS) spectroscopy provides elemental and chemical state information.

Purpose of the Study:

  • To develop a new 3D Micro-XAFS spectroscopy approach for non-destructive, depth-resolved chemical speciation.
  • To address spectral distortions inherent in fluorescence mode measurements of stratified samples.
  • To validate a reconstruction algorithm for accurate speciation analysis in superficial and in-depth layers.

Main Methods:

  • Integration of 3D Micro X-ray Fluorescence Spectroscopy (3D Micro-XRF) with conventional X-ray Absorption Fine Structure Spectroscopy (XAFS).
  • Development and validation of a reconstruction algorithm to correct for absorption effects in fluorescence spectra.
  • Calculation of analyte attenuation coefficients for successive layers within stratified materials.

Main Results:

  • Successful development of a novel 3D Micro-XAFS spectroscopy technique.
  • Demonstration of a reliable reconstruction algorithm for obtaining undistorted spectra from stratified samples.
  • Validation of the method for non-destructive, three-dimensionally resolved chemical speciation.

Conclusions:

  • The developed 3D Micro-XAFS spectroscopy offers a powerful new tool for analyzing stratified materials.
  • The reconstruction algorithm effectively overcomes limitations of fluorescence mode measurements.
  • This technique facilitates advanced, non-destructive chemical speciation with depth resolution.