Related Experiment Video

Updated: May 25, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

[Expression, purification and protective antigen analysis of cell wall protein MRP of Streptococcus suis type 2]

Ping-ping Wang1, Ya-ya Pian, Yuan Yuan

  • 1Sports School, Shaanxi Normal University, Xi'an, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|February 7, 2012
PubMed
Abstract

Insights

The study identified the mrp gene product from Streptococcus suis as a key protective antigen. This recombinant protein, MRP, demonstrated significant immunoprotective capabilities in animal models, highlighting its potential for vaccine development.

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Streptococcus suis type 2 is a significant pathogen.
  • The mrp gene product's role as a protective antigen requires further investigation.

Purpose of the Study:

  • Amplify and express the mrp gene from Streptococcus suis type 2 05ZYH33.
  • Produce high-purity recombinant MRP protein in E. coli BL21.
  • Evaluate the protective antigen properties of recombinant MRP.

Main Methods:

  • PCR amplification and cloning of the mrp gene into pET28a(+).
  • Expression in E. coli BL21 and purification of recombinant MRP via affinity chromatography.
  • Generation of anti-serum in rabbits and evaluation using ELISA and opsonophagocytic killing tests.

Main Results:

  • High-purity recombinant MRP protein was successfully expressed and purified.
  • Immunization with recombinant MRP significantly improved survival rates in CD-1 mice.
  • Anti-serum against MRP reduced the survival of wild-type Streptococcus suis 05ZYH33 in blood, but not the MRP mutant.

Conclusions:

  • The mrp gene product (MRP) exhibits significant protective antigen activity.
  • MRP is a potential target for developing vaccines against Streptococcus suis type 2 infections.

More Related Videos

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP009208:53

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

Related Concept Videos

Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Special Staining Techniques01:13

Special Staining Techniques

Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...