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DIGE: past and future.

Jonathan S Minden1

  • 1Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA. minden@cmu.edu

Methods in Molecular Biology (Clifton, N.J.)
|February 8, 2012
PubMed
Summary
This summary is machine-generated.

Difference gel electrophoresis (DIGE) has evolved significantly since its beginning. This chapter traces its journey from initial development through commercialization and ongoing advancements.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Analytical Chemistry

Background:

  • Difference gel electrophoresis (DIGE) is a key technique in proteomics.
  • Understanding its historical development is crucial for appreciating its current applications.

Purpose of the Study:

  • To provide a historical overview of difference gel electrophoresis (DIGE).
  • To trace the evolution of DIGE from its inception to its commercialization and beyond.

Main Methods:

  • Historical review of scientific literature.
  • Analysis of key milestones in DIGE development.
  • Examination of commercialization processes.

Main Results:

  • The chapter details the foundational concepts that led to DIGE.
  • It highlights critical advancements and innovations in the technique.
  • The transition from laboratory development to commercial availability is discussed.

Conclusions:

  • Difference gel electrophoresis (DIGE) has a rich developmental history.
  • Its journey reflects significant progress in proteomic analysis technologies.