[The electrophoretic cell analysis applying the instrument complex "Cito-expert"].

Area of Science:

• Biophysics
• Cell Biology
• Electrophoresis

Context:

• Cell membrane Z-potential and electric discharge are key indicators of cellular homeostasis.
• Cell electrophoresis is a crucial technique for measuring these cellular properties.
• Existing methods lack efficiency and comprehensive analysis capabilities.

Purpose:

• To introduce the first national serial instrument complex for cell electrophoresis.
• To present a novel device utilizing an alternating electric field and an original electrophoretic camera.
• To enable precise determination of cell oscillation amplitude, effective charge, and cell distribution.

Summary:

• The developed instrument complex analyzes cell oscillation amplitude, directly proportional to effective charge, in an alternating electric field.
• It quantifies the proportion of active cells, their distribution patterns, and statistical measures like asymmetry and excess.
• Analysis is rapid (5-8 min), requires minimal sample volume (40 µL), and processes 100-300 cells per run.

Impact:

• Facilitates diagnosis and monitoring of intoxication-related critical conditions.
• Enables rapid assessment of liquid medium toxicity and nanomaterial bioactivity at the cellular level.
• Supports prompt screening of pharmacological substances and pharmaceuticals, expanding cell electrophoresis applications.