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Related Concept Videos

Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Group I intron ribozymes.

Henrik Nielsen1

  • 1Department of Cellular and Molecular Medicine, The Panum Institute, University of Copenhagen, Copenhagen, Denmark. hamra@sund.ku.dk

Methods in Molecular Biology (Clifton, N.J.)
|February 9, 2012
PubMed
Summary
This summary is machine-generated.

Group I intron ribozymes are versatile RNA enzymes with diverse structures and functions. This study offers methods for characterizing new group I intron ribozymes and their biological roles.

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • Group I intron ribozymes are key RNA enzymes with significant roles in RNA biology.
  • They exhibit structural and catalytic diversity, unlike other ribozyme classes.
  • Known pathways include splicing and circularization, yielding various RNA products.

Purpose of the Study:

  • To provide a strategy and protocols for the initial characterization of novel group I intron ribozymes.
  • To explore the diverse biological phenomena associated with group I introns and their products.
  • To facilitate the study of RNA enzymes and their functions.

Main Methods:

  • Development of a strategic approach for analyzing new ribozymes.
  • Establishment of protocols for initial characterization.
  • Comparative analysis of structural and catalytic variations.

Main Results:

  • A framework for characterizing group I intron ribozymes is presented.
  • The study highlights the potential of intronic products in target integration and circular RNA formation.
  • Demonstrates the rich biological potential of group I introns.

Conclusions:

  • Group I intron ribozymes represent a rich source of biological phenomena due to their versatility.
  • The provided strategy and protocols enable the initial characterization of newly discovered group I intron ribozymes.
  • Further research into these ribozymes can advance RNA biology and enzyme development.