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Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...

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Related Experiment Video

Updated: May 25, 2026

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

Deconstructing the centriole: structure and number control.

Daniela A Brito1, Susana Montenegro Gouveia, Mónica Bettencourt-Dias

  • 1Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, P-2780-156 Oeiras, Portugal.

Current Opinion in Cell Biology
|February 11, 2012
PubMed
Summary
This summary is machine-generated.

Centriole assembly, crucial for cell function, is poorly understood. Recent advances in imaging and biochemical techniques are revealing the mechanisms behind this complex organelle

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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Related Experiment Videos

Last Updated: May 25, 2026

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Imaging Centrosomes in Fly Testes
09:41

Imaging Centrosomes in Fly Testes

Published on: September 20, 2013

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • Centrioles are vital microtubule-based organelles essential for centrosome, cilia, and flagella assembly.
  • These structures play critical roles in diverse cellular and developmental processes.
  • Despite being discovered nearly a century ago, centriole assembly mechanisms remain largely unknown.

Purpose of the Study:

  • To review recent functional studies on centriole biogenesis.
  • To discuss the contribution of various molecular players in centriole assembly.
  • To highlight the impact of advanced imaging and biochemical techniques on understanding centriole structure and function.

Main Methods:

  • Review of forefront functional studies in centriole biogenesis.
  • Analysis of data from sensitive structural, imaging, and biochemical techniques.
  • Discussion of emerging super-resolution microscopy applications.

Main Results:

  • Recent functional studies have identified key players in centriole biogenesis.
  • Advanced techniques are providing insights into the assembly of this complex organelle.
  • Super-resolution analyses are poised to significantly advance the field.

Conclusions:

  • Understanding centriole assembly is crucial for comprehending cellular and developmental processes.
  • Technological advancements are rapidly improving our knowledge of centriole biogenesis.
  • The field of centriole research is entering an exciting new phase driven by super-resolution imaging.