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Related Experiment Video

Updated: May 25, 2026

Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells
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Microfluidic Approach to Resolve Simultaneous and Sequential Cytokine Secretion of Individual Polyfunctional Cells

Published on: March 8, 2024

Droplet microfluidics for single-cell analysis.

Eric Brouzes1

  • 1Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USA. ebrouzes@gmail.com

Methods in Molecular Biology (Clifton, N.J.)
|February 11, 2012
PubMed
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This guide details developing droplet-based workflows for single-cell analysis using stable fluorinated surfactant systems. It covers formulation, circuit design, microfluidic chip fabrication, and fluorescence detection for efficient cell encapsulation and analysis.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Droplet microfluidics is crucial for single-cell analysis.
  • Stable droplet formation relies heavily on the surfactant system.
  • Fluorinated surfactants offer superior droplet stability and material retrieval.

Purpose of the Study:

  • To provide a comprehensive overview of developing droplet-based workflows for single-cell analysis.
  • To detail the critical aspects of surfactant system selection and microfluidic chip design.
  • To review experimental setups for fluorescence detection in droplet microfluidics.

Main Methods:

  • Focus on fluorinated surfactant systems for droplet stabilization.
  • Discusses experimental parameters influencing surfactant choice and formulation.

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  • Presents recipes for circuit design, microfluidic chip fabrication, and surface treatment.
  • Reviews experimental setups for fluorescence detection, cell injection, and incubation.

    • Main Results:

    • Fluorinated surfactant systems enable highly stable droplets.
    • Optimized formulation and fabrication are key to successful droplet-based assays.
    • Integrated droplet modules can be designed for specific workflows.
    • Effective cell injection and incubation protocols are essential for reliable analysis.

    Conclusions:

    • Developing droplet-based workflows for single-cell analysis requires careful consideration of surfactant systems, microfluidic design, and experimental setup.
    • Fluorinated surfactants are a critical component for achieving stable droplets and efficient material recovery.
    • This chapter provides a practical guide for researchers entering the field of droplet microfluidics for single-cell applications.