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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Determining the Plane of Cell Division

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Updated: May 25, 2026

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

Cell sorting by deterministic cell rolling.

Sungyoung Choi¹, Jeffrey M Karp, Rohit Karnik

¹Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

|February 14, 2012

Summary

This summary is machine-generated.

Deterministic cell rolling uses transient molecular interactions to efficiently sort cells in one step. This novel method achieves high purity, offering a significant advancement in cell separation technology.

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Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns

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Published on: February 13, 2011

Area of Science:

Biotechnology
Cell Biology
Biophysics

Background:

Cell sorting is crucial for research and therapeutics.
Existing methods face challenges in purity, efficiency, or throughput.
Transient cell-surface molecular interactions are fundamental to cell adhesion and migration.

Purpose of the Study:

To introduce and define the concept of "deterministic cell rolling" for cell separation.
To demonstrate the potential of this technique for high-purity, single-step cell sorting.
To highlight the mechanism based on transient molecular interactions.

Main Methods:

Leveraging transient cell-surface molecular interactions.
Utilizing the principles of cell rolling for separation.
Implementing a single-step sorting process.

Main Results:

Achieved high purity in cell sorting.
Demonstrated high efficiency in cell separation.
Successfully sorted cells in a single step.

Conclusions:

Deterministic cell rolling offers a novel and effective approach for cell separation.
The technique shows promise for various applications requiring pure cell populations.
Transient molecular interactions can be precisely controlled for deterministic cell sorting.