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The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection
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Single-cell electroporation using proton beam fabricated biochips.

S Homhuan1, B Zhang, F-S Sheu

  • 1Prince of Songkla University, Department of Physics, Hat Yai, Songkhla 90112, Thailand. hsureerat@hotmail.com

Biomedical Microdevices
|February 14, 2012
PubMed
Summary

This study introduces a novel biochip for single cell electroporation using proton beam writing. The device achieves high transfection rates and cell viability, offering a promising method for intracellular delivery.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Cell Biology

Background:

  • Electroporation is a key method for introducing molecules into cells.
  • Existing methods face challenges in precision and efficiency for single-cell applications.
  • Novel fabrication techniques are needed for advanced cellular manipulation tools.

Purpose of the Study:

  • To design and fabricate a novel single cell electroporation biochip.
  • To investigate the efficiency of this biochip for intracellular delivery.
  • To optimize electroporation parameters for high transfection and viability.

Main Methods:

  • Fabrication of a biochip with high aspect ratio nickel micro-electrodes using Proton Beam Writing (PBW).
  • Attachment of individual cells between micro-electrodes for targeted manipulation.
  • Application of electrical impulses to induce electroporation and facilitate molecule uptake (SYTOX® Green nucleic acid stain).
  • Investigation of electric field strength, pulse duration, and pulse number effects.

Main Results:

  • Successful incorporation of SYTOX® Green nucleic acid stain into mouse neuroblastoma (N2a) cells.
  • Achieved high transfection rates.
  • Maintained high cell viability at 82.1% for transfection and 86.7% overall.
  • Demonstrated the potential for delivering various molecules like fluorophores, nanoparticles, and proteins.

Conclusions:

  • The developed single cell electroporation biochip is effective for precise intracellular delivery.
  • Proton Beam Writing enables the fabrication of high-quality microstructures for cell manipulation.
  • This technology shows significant promise for advancing cellular research and therapeutic applications.