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Updated: May 24, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
[Biocatalytic synthesis of pharmacology perspective stigmast-4-en-3-one using Rhodococci cells]
Abstract:
The conditions for a directed biocatalytic oxidation of beta-sitosterol to a pharmacologically promising stigmast-4-en-3-one using Rhodococcus actinobacteria were selected. It was shown that palmitic acid induced the cholesterol oxidase reaction and allowed for the decrease in the bioconversion process duration from 7 to 5 days. The maximum level ofstigmast-4-ene-3-one formation was achieved using n-hexadecane as an additional growth substrate. With increased concentrations of beta-sitosterol (up to 2 g/L) an effective target product formation (80%) was achieved in the presence of Tween-80 and beta-cyclodextrine. R. erythropolis strains were 1.5-2 times more active than R. ruber strains in catalyzing the beta-sitosterol biotransformation process.
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