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Updated: May 24, 2026

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))
04:48

Setup of Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry (CE-ICP-MS) for Quantification of Iron Redox Species (Fe(II), Fe(III))

Published on: May 4, 2020

[In vitro effect of iron overload on bone marrow cell function by inducing the reactive oxygen species]

Fang Xie1, Ming-feng Zhao, Yu-ming Li

  • 1Tianjin First Central Hospital, Tianjin Medical University, Tianjin 300192, China.

Zhonghua Xue Ye Xue Za Zhi = Zhonghua Xueyexue Zazhi
|February 17, 2012
PubMed
Abstract

Insights

Iron overload increases reactive oxygen species (ROS) and impairs bone marrow cell function, affecting hematopoiesis. This damage can be reversed by removing excess iron and ROS.

Area of Science:

  • Hematology
  • Cell Biology
  • Toxicology

Context:

  • Iron overload is a condition where excess iron accumulates in the body.
  • This accumulation can lead to cellular damage and dysfunction.
  • Bone marrow is critical for hematopoiesis, the process of blood cell formation.

Purpose:

  • To investigate the in vitro effects of iron overload on bone marrow mononuclear cells (BMMNCs).
  • To assess the impact of iron overload on reactive oxygen species (ROS) generation and BMMNC function.
  • To evaluate the potential of deferasirox (DFO) and N-acetyl-L-cysteine (NAC) in mitigating iron overload-induced damage.

Summary:

  • Culturing BMMNCs with ferric citrate (FAC) increased labile iron pool (LIP) in a time- and concentration-dependent manner.
  • Iron overload significantly elevated ROS levels, increased apoptosis, and reduced hematopoietic colony formation and CD34+ cell percentage.
  • Treatment with DFO or NAC effectively reduced ROS and reversed the negative effects on BMMNCs.

Impact:

  • Iron overload adversely affects hematopoiesis through ROS generation.
  • Therapeutic strategies targeting iron chelation and ROS reduction can correct these hematological impairments.
  • Findings may inform treatments for dyshematopoiesis in iron overload conditions.

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