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Updated: May 24, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Investigate the impact of hepatitis B virus infection on sperm DNA integrity]
Jian-Wei Chen1, Yun Cui, Xiao-Xia Zhang
1Ningbo Traditional Chinese Medicine Attached to Zhejiang Traditional Chinese Medicine University, Ningbo 351012, China. cjw4015@sina.com
Objective:
Investigated the impact of HBV infection on sperm DNA integrity and its applied significance.
Methods:
12 cases infertility male with HBV infection in patient group and 11 cases of normal male without HBV infection in the control group were by SCD and CASA.
Results:
The DFI in the patient group was (23.4 +/- 10.2)%, while it was in the control group was (11.6 +/- 5.9)%. The difference of the DFI in these two groups was significantly, P < 0.01. The sperm concentration and forward motility (a + b) in the patient group were lower than that in the control group, P < 0.01. There were also significantly of the normal sperm and sperm activity between the two groups.
Conclusion:
HBV infection not only affected the conventional semen parameters, but also caused serious damage on sperm DNA.
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