Simultaneous determination of ascorbic acid, dopamine and uric acid using high-performance screen-printed graphene...

Area of Science:

• Electrochemistry
• Materials Science
• Analytical Chemistry

Background:

• Graphene's unique properties, including a wide potential window and fast electron transfer kinetics, make it suitable for electrochemical sensing.
• Disposable electrodes offer convenience and reduced contamination risk in analytical applications.
• Simultaneous determination of biologically relevant molecules like ascorbic acid, dopamine, and uric acid is crucial for various diagnostic and research purposes.

Purpose of the Study:

• To develop a disposable and sensitive screen-printed electrode utilizing graphene-based ink.
• To evaluate the electrocatalytic activity of the developed screen-printed graphene electrode (SPGNE) for ascorbic acid (AA), dopamine (DA), and uric acid (UA).
• To establish a method for the simultaneous determination of AA, DA, and UA in a ternary mixture using differential pulse voltammetry.

Main Methods:

• Fabrication of a screen-printed electrode using graphene ink.
• Electrochemical characterization of the SPGNE.
• Differential pulse voltammetry (DPV) for simultaneous detection of AA, DA, and UA.
• Analysis of real biological samples to assess sensor feasibility.

Main Results:

• The SPGNE exhibited excellent electrocatalytic activity for the oxidation of AA, DA, and UA, with well-defined anodic peaks.
• Simultaneous determination using DPV achieved linear response ranges of 4.0–4500 μM (AA), 0.5–2000 μM (DA), and 0.8–2500 μM (UA).
• Low detection limits were achieved: 0.95 μM (AA), 0.12 μM (DA), and 0.20 μM (UA) (S/N=3).
• The SPGNE demonstrated high reproducibility, stability, and effectiveness in analyzing biological samples.

Conclusions:

• The developed disposable SPGNE is a sensitive and selective sensor for the simultaneous determination of AA, DA, and UA.
• The electrode's performance characteristics make it a promising alternative for routine sensing applications in biological sample analysis.
• This graphene-based sensor offers a practical solution for electrochemical monitoring of key biomolecules.